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nebnext ultratm ii directional rna library prep  (New England Biolabs)


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    New England Biolabs nebnext ultratm ii directional rna library prep
    Nebnext Ultratm Ii Directional Rna Library Prep, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 647 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+sample+purification+beads/NEBNext+Ultra+II+Directional+RNA+Library+Prep+with+Sample+Purification+Beads/bio_rxiv__64898__2026__03__12__711491-335-26-26
    Average 97 stars, based on 647 article reviews
    nebnext ultratm ii directional rna library prep - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Bacteria:

    Article Title: High quality transcriptome profiling confirms the transcriptional landscape of Treponema pallidum subsp. pallidum.
    Article Snippet: The “co-culture” RNA sample was treated with an NEBNext® Poly(A) mRNA Magnetic Isolation kit (New England Biolabs, UK), and 100 μl of the produced supernatant (bacterial fraction) was used in the subsequent workflow. .. Ribosomal RNA was depleted using NEBNext® rRNA Depletion Kit (Bacteria) and NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) with RNA Sample Purification Beads (New England Biolabs, UK). ..

    Article Title: Spatial metabolomics reveals the role of penicillic acid in cheese-rind microbiome disruption by a spoilage fungus.
    Article Snippet: .. Bacterial rRNA was removed using the NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads (NEB #E7860). .. Fungal rRNA depletion was achieved using a custom oligonucleotide pool (2 μM final concentration per probe; Integrated DNA Technologies) targeting A. westerdijkiae rRNA, used in conjunction with the NEBNext RNA Depletion Core Reagent Set (NEB #E7865).

    Article Title: Ecophysiology and niche differentiation of three genera of polyphosphate-accumulating bacteria in a full-scale wastewater treatment plant
    Article Snippet: RNA concentration and quality were checked using Qubit 3.0 fluorometer (Thermo Fisher Scientific, MA, USA) and Agilent TapeStation RNA ScreenTapes ( ). .. Library preparation was performed using NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads, followed by NEBNext Ultra II Directional RNA library preparation (New England Biolabs), based on the manufacturer’s recommendations. .. Library concentration and quality control were performed using Qubit 3.0 fluorometer (Thermo Fisher Scientific, MA, USA) and Agilent TapeStation High Sensitivity D1000 ScreenTapes ( ).

    Article Title: Spatial metabolomics reveals the role of penicillic acid in cheese-rind microbiome disruption by a spoilage fungus
    Article Snippet: .. Bacterial rRNA was removed using the NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads (NEB #E7860). .. Fungal rRNA depletion was achieved using a custom oligonucleotide pool (2 μM final concentration per probe; Integrated DNA Technologies) targeting A. westerdijkiae rRNA, used in conjunction with the NEBNext RNA Depletion Core Reagent Set (NEB #E7865).

    Article Title: Respiratory virus infection dynamics and genomic surveillance to detect seasonal influenza subtypes in wastewater: A longitudinal study in Bengaluru, India
    Article Snippet: RNA integrity was analyzed using a 4200 Tape Station (Agilent) and an RNA Screen Tape assay kit. .. To enrich the viral RNA content before library preparation, ribosomal RNA (rRNA) was depleted using a combination of two kits: NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (Cat No. NEB E7405) and NEBNext rRNA Depletion Kit (Bacteria) (Cat No. NEB E7860), along with RNA Sample Purification Beads (Cat No. NEB #E7405) as per the manufacturer’s protocols, starting with a total RNA concentration of 100 ng - 1μg. rRNA-depleted RNA libraries were prepared using NEBNext Ultra II Directional RNA Library Prep (Cat. No. E7765L) with Sample Purification Beads and sequenced on the NovaSeq 6000 platform using an S4 flow cell and a 2x150 bp read length kit (Illumina). .. Libraries were prepared using the Illumina COVIDSeq Test kit (Cat No: 20043675, Illumina Inc, USA).

    Article Title: Bioelectrocatalytic conversion of CO₂ to PHA bioplastics using engineered methylotrophs
    Article Snippet: .. Sequencing, alignment, and read count matrix generation Library preparation was performed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760) and NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads (E7860). ..

    Article Title: Spatial Metabolomics Reveals the Role of Penicillic Acid in Cheese Rind Microbiome Disruption by a Spoilage Fungus
    Article Snippet: .. Bacterial rRNA was removed using the NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads (NEB #E7860). .. Fungal rRNA depletion was achieved using a custom oligonucleotide pool (2 μM final concentration per probe; Integrated DNA Technologies) targeting A. westerdijkiae rRNA, used in conjunction with the NEBNext RNA Depletion Core Reagent Set (NEB #E7865).

    Sample Purification:

    Article Title: High quality transcriptome profiling confirms the transcriptional landscape of Treponema pallidum subsp. pallidum.
    Article Snippet: The “co-culture” RNA sample was treated with an NEBNext® Poly(A) mRNA Magnetic Isolation kit (New England Biolabs, UK), and 100 μl of the produced supernatant (bacterial fraction) was used in the subsequent workflow. .. Ribosomal RNA was depleted using NEBNext® rRNA Depletion Kit (Bacteria) and NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) with RNA Sample Purification Beads (New England Biolabs, UK). ..

    Article Title: Engineered ADARs enable single-nucleotide resolution DNA A-to-G editing without bystander effects
    Article Snippet: For targeted RNA sequencing , cDNA was synthesized using ReverTra Ace (Toyobo, FSK-101F) according to the manufacturer’s protocol and sequenced using Illumina MiniSeq as previously described. .. For whole-RNA sequencing , total RNA was processed using the NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) and RNA Sample Purification Beads (NEB, E7405L), followed by library preparation using the NEBNext® UltraTM II RNA Library Prep Kit with Sample Purification Beads (NEB, E7775S), according to the manufacturer’s instructions. .. Sequencing was outsourced to Novogene (NovaSeq X Plus), with 6 Gb of sequencing data generated per sample.

    Article Title: Spatial metabolomics reveals the role of penicillic acid in cheese-rind microbiome disruption by a spoilage fungus.
    Article Snippet: .. Bacterial rRNA was removed using the NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads (NEB #E7860). .. Fungal rRNA depletion was achieved using a custom oligonucleotide pool (2 μM final concentration per probe; Integrated DNA Technologies) targeting A. westerdijkiae rRNA, used in conjunction with the NEBNext RNA Depletion Core Reagent Set (NEB #E7865).

    Article Title: Ecophysiology and niche differentiation of three genera of polyphosphate-accumulating bacteria in a full-scale wastewater treatment plant
    Article Snippet: RNA concentration and quality were checked using Qubit 3.0 fluorometer (Thermo Fisher Scientific, MA, USA) and Agilent TapeStation RNA ScreenTapes ( ). .. Library preparation was performed using NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads, followed by NEBNext Ultra II Directional RNA library preparation (New England Biolabs), based on the manufacturer’s recommendations. .. Library concentration and quality control were performed using Qubit 3.0 fluorometer (Thermo Fisher Scientific, MA, USA) and Agilent TapeStation High Sensitivity D1000 ScreenTapes ( ).

    Article Title: Spatial metabolomics reveals the role of penicillic acid in cheese-rind microbiome disruption by a spoilage fungus
    Article Snippet: .. Bacterial rRNA was removed using the NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads (NEB #E7860). .. Fungal rRNA depletion was achieved using a custom oligonucleotide pool (2 μM final concentration per probe; Integrated DNA Technologies) targeting A. westerdijkiae rRNA, used in conjunction with the NEBNext RNA Depletion Core Reagent Set (NEB #E7865).

    Article Title: Respiratory virus infection dynamics and genomic surveillance to detect seasonal influenza subtypes in wastewater: A longitudinal study in Bengaluru, India
    Article Snippet: RNA integrity was analyzed using a 4200 Tape Station (Agilent) and an RNA Screen Tape assay kit. .. To enrich the viral RNA content before library preparation, ribosomal RNA (rRNA) was depleted using a combination of two kits: NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (Cat No. NEB E7405) and NEBNext rRNA Depletion Kit (Bacteria) (Cat No. NEB E7860), along with RNA Sample Purification Beads (Cat No. NEB #E7405) as per the manufacturer’s protocols, starting with a total RNA concentration of 100 ng - 1μg. rRNA-depleted RNA libraries were prepared using NEBNext Ultra II Directional RNA Library Prep (Cat. No. E7765L) with Sample Purification Beads and sequenced on the NovaSeq 6000 platform using an S4 flow cell and a 2x150 bp read length kit (Illumina). .. Libraries were prepared using the Illumina COVIDSeq Test kit (Cat No: 20043675, Illumina Inc, USA).

    Article Title: Bioelectrocatalytic conversion of CO₂ to PHA bioplastics using engineered methylotrophs
    Article Snippet: .. Sequencing, alignment, and read count matrix generation Library preparation was performed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760) and NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads (E7860). ..

    Article Title: Spatial Metabolomics Reveals the Role of Penicillic Acid in Cheese Rind Microbiome Disruption by a Spoilage Fungus
    Article Snippet: .. Bacterial rRNA was removed using the NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads (NEB #E7860). .. Fungal rRNA depletion was achieved using a custom oligonucleotide pool (2 μM final concentration per probe; Integrated DNA Technologies) targeting A. westerdijkiae rRNA, used in conjunction with the NEBNext RNA Depletion Core Reagent Set (NEB #E7865).

    Sequencing:

    Article Title: Engineered ADARs enable single-nucleotide resolution DNA A-to-G editing without bystander effects
    Article Snippet: For targeted RNA sequencing , cDNA was synthesized using ReverTra Ace (Toyobo, FSK-101F) according to the manufacturer’s protocol and sequenced using Illumina MiniSeq as previously described. .. For whole-RNA sequencing , total RNA was processed using the NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) and RNA Sample Purification Beads (NEB, E7405L), followed by library preparation using the NEBNext® UltraTM II RNA Library Prep Kit with Sample Purification Beads (NEB, E7775S), according to the manufacturer’s instructions. .. Sequencing was outsourced to Novogene (NovaSeq X Plus), with 6 Gb of sequencing data generated per sample.

    Article Title: Bioelectrocatalytic conversion of CO₂ to PHA bioplastics using engineered methylotrophs
    Article Snippet: .. Sequencing, alignment, and read count matrix generation Library preparation was performed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760) and NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads (E7860). ..

    RNA Library Preparation:

    Article Title: Ecophysiology and niche differentiation of three genera of polyphosphate-accumulating bacteria in a full-scale wastewater treatment plant
    Article Snippet: RNA concentration and quality were checked using Qubit 3.0 fluorometer (Thermo Fisher Scientific, MA, USA) and Agilent TapeStation RNA ScreenTapes ( ). .. Library preparation was performed using NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads, followed by NEBNext Ultra II Directional RNA library preparation (New England Biolabs), based on the manufacturer’s recommendations. .. Library concentration and quality control were performed using Qubit 3.0 fluorometer (Thermo Fisher Scientific, MA, USA) and Agilent TapeStation High Sensitivity D1000 ScreenTapes ( ).

    Concentration Assay:

    Article Title: Respiratory virus infection dynamics and genomic surveillance to detect seasonal influenza subtypes in wastewater: A longitudinal study in Bengaluru, India
    Article Snippet: RNA integrity was analyzed using a 4200 Tape Station (Agilent) and an RNA Screen Tape assay kit. .. To enrich the viral RNA content before library preparation, ribosomal RNA (rRNA) was depleted using a combination of two kits: NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (Cat No. NEB E7405) and NEBNext rRNA Depletion Kit (Bacteria) (Cat No. NEB E7860), along with RNA Sample Purification Beads (Cat No. NEB #E7405) as per the manufacturer’s protocols, starting with a total RNA concentration of 100 ng - 1μg. rRNA-depleted RNA libraries were prepared using NEBNext Ultra II Directional RNA Library Prep (Cat. No. E7765L) with Sample Purification Beads and sequenced on the NovaSeq 6000 platform using an S4 flow cell and a 2x150 bp read length kit (Illumina). .. Libraries were prepared using the Illumina COVIDSeq Test kit (Cat No: 20043675, Illumina Inc, USA).



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